Lowenstein-Jensen is a medium enriched with egg, potato, or serum commonly used to cultivate mycobacterium.
Members of the genus Mycobacterium act as the causative agents for tuberculosis and leprosy. Mycobacterium contain an abundance of fats and waxes in their cell walls, including mycolic acid.
Acid-Fast Technique — Mixed
Positive (+) result— Acid-fast organisms will be red because carbolfuchsin stain will adhere to acid-fast bacterium.
carbolfuchsin — the primary dye.
acid-alcohol — decolorizer.
methylene blue — the secondary dye.
